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lunascript rt master mix kit  (New England Biolabs)


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    Structured Review

    New England Biolabs lunascript rt master mix kit
    Lunascript Rt Master Mix Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lunascript+rt+master+mix+kit/LunaScript+RT+Master+Mix/pm42034051-708-11-16
    Average 97 stars, based on 136 article reviews
    lunascript rt master mix kit - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: Nuclear envelope budding enables export of large transcripts in muscle cells.
    Article Snippet: RNA was eluted in 50 μl of nuclease-free water and the concentration was determined using a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific). .. First-strand cDNA synthesis was performed on all RNA samples using the LunaScript® RT Master Mix Kit (NEB #E3025L) combined with the Random Primer Mix (NEB #S1330S) according to the manufacturer’s included protocol. ..

    Article Title: De novo recovery of Ghana virus, an African bat Henipavirus, reveals differential tropism and attenuated pathogenicity compared to Nipah virus
    Article Snippet: .. The Primer-free LunaScript RT Master Mix Kit (New England Biolabs) was used for first-strand cDNA synthesis using gene-specific primers targeting EFNB2, EFNB3, or GAPDH. ..

    Article Title: De novo recovery of Ghana virus, an African bat Henipavirus, reveals differential tropism and attenuated pathogenicity compared to Nipah virus.
    Article Snippet: Total RNA was extracted from respective bat cell lines using TRIzol reagent and the Direct-zol RNA Miniprep kit (Zymo Research). .. RNA was eluted in ultra-pure water and stored at − 80 ◦ C. The Primer-free LunaScript RT Master Mix Kit (New England Biolabs) was used for first-strand cDNA synthesis using gene-specific primers targeting EFNB2, EFNB3, or GAPDH. ..

    other:

    Article Title: SARS-CoV-2 Nsp15 facilitates immune evasion and viral replication by limiting multiple host innate immune pathways, including cGAS-STING.
    Article Snippet: Mouse cGAS and STING coding sequences fused with N- terminal HA tag and C- terminal V5 tag were synthesized by Twist Bioscience and further cloned into the pcDNA3.1 vector.

    Article Title: Enhancers that direct gene expression to central nervous system vascular endothelial cells in vivo.
    Article Snippet: Biotinylated isolectin GS-IB4 (GSL; Thermo Fisher, I21414) and streptavidin magnetic beads were used to further purify the brain vascular fragments.

    Incubation:

    Article Title: Inositol hexakisphosphate Functions as a Cofactor and Modulator of ADAR1 Activity
    Article Snippet: .. Then, RNase Inhibitor RiboLock (1,6 U/μl) was added and 25 μL incubated with ADAR-IVTra (IP 6 , IP 5 or no IPs) protein bound magnetic beads at 27 °C for 24 h. For RT-PCR, 4 ng of IVT RNA were in vitro transcribed using the reverse primer of the respectively gene (Table S6) and QuantiTect Reverse Transcription Kit (Qiagen) or LunaScript RT Master Mix Kit (Cat. # E3025L, New England Biolabs) according to the manufacturer’s protocol. cDNA amplification was performed using the respectively primers from Table S6 and DreamTaq Green PCR Master Mix (2x) (Thermo Fisher Scientific). .. PCR product was purified with GeneJET PCR Purification Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol and Sanger sequenced using standard T7 primer (5’-TAATACGACTCACTATAGGG-3′, Seqlab, Microsynth) or the reverse primer in the case of HTR2C (Table S6).

    Magnetic Beads:

    Article Title: Inositol hexakisphosphate Functions as a Cofactor and Modulator of ADAR1 Activity
    Article Snippet: .. Then, RNase Inhibitor RiboLock (1,6 U/μl) was added and 25 μL incubated with ADAR-IVTra (IP 6 , IP 5 or no IPs) protein bound magnetic beads at 27 °C for 24 h. For RT-PCR, 4 ng of IVT RNA were in vitro transcribed using the reverse primer of the respectively gene (Table S6) and QuantiTect Reverse Transcription Kit (Qiagen) or LunaScript RT Master Mix Kit (Cat. # E3025L, New England Biolabs) according to the manufacturer’s protocol. cDNA amplification was performed using the respectively primers from Table S6 and DreamTaq Green PCR Master Mix (2x) (Thermo Fisher Scientific). .. PCR product was purified with GeneJET PCR Purification Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol and Sanger sequenced using standard T7 primer (5’-TAATACGACTCACTATAGGG-3′, Seqlab, Microsynth) or the reverse primer in the case of HTR2C (Table S6).

    In Vitro:

    Article Title: Inositol hexakisphosphate Functions as a Cofactor and Modulator of ADAR1 Activity
    Article Snippet: .. Then, RNase Inhibitor RiboLock (1,6 U/μl) was added and 25 μL incubated with ADAR-IVTra (IP 6 , IP 5 or no IPs) protein bound magnetic beads at 27 °C for 24 h. For RT-PCR, 4 ng of IVT RNA were in vitro transcribed using the reverse primer of the respectively gene (Table S6) and QuantiTect Reverse Transcription Kit (Qiagen) or LunaScript RT Master Mix Kit (Cat. # E3025L, New England Biolabs) according to the manufacturer’s protocol. cDNA amplification was performed using the respectively primers from Table S6 and DreamTaq Green PCR Master Mix (2x) (Thermo Fisher Scientific). .. PCR product was purified with GeneJET PCR Purification Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol and Sanger sequenced using standard T7 primer (5’-TAATACGACTCACTATAGGG-3′, Seqlab, Microsynth) or the reverse primer in the case of HTR2C (Table S6).

    Reverse Transcription:

    Article Title: Inositol hexakisphosphate Functions as a Cofactor and Modulator of ADAR1 Activity
    Article Snippet: .. Then, RNase Inhibitor RiboLock (1,6 U/μl) was added and 25 μL incubated with ADAR-IVTra (IP 6 , IP 5 or no IPs) protein bound magnetic beads at 27 °C for 24 h. For RT-PCR, 4 ng of IVT RNA were in vitro transcribed using the reverse primer of the respectively gene (Table S6) and QuantiTect Reverse Transcription Kit (Qiagen) or LunaScript RT Master Mix Kit (Cat. # E3025L, New England Biolabs) according to the manufacturer’s protocol. cDNA amplification was performed using the respectively primers from Table S6 and DreamTaq Green PCR Master Mix (2x) (Thermo Fisher Scientific). .. PCR product was purified with GeneJET PCR Purification Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol and Sanger sequenced using standard T7 primer (5’-TAATACGACTCACTATAGGG-3′, Seqlab, Microsynth) or the reverse primer in the case of HTR2C (Table S6).

    Article Title: ER-associated control of axonal vesicle trafficking during neuronal development
    Article Snippet: RNA quantity and purity were assessed by spectrophotometry (NanoDrop, Thermo Fisher Scientific). .. For each sample, 500 ng to 1 μg of RNA was reverse-transcribed using the LunaScript RT Master Mix Kit (New England Biolabs, E3025L), in a final reaction volume of 20 μL, according to the supplier’s recommendations. qPCR was performed using the Luna Universal qPCR Master Mix (New England Biolabs, M3003X) on a 7500 qPCR fast System (Applied Biosystems). .. Each 20 μL reaction contained 4 μL of diluted cDNA and gene-specific primers purchased from IDT DNA: Rat ActinB: Rn.PT.39a.22214838.g, Rat Lppr4 (PRG1): Rn.PT.58.19098303, Rat Lppr3 (PRG2): Rn.PT.58.35042039.

    Amplification:

    Article Title: Inositol hexakisphosphate Functions as a Cofactor and Modulator of ADAR1 Activity
    Article Snippet: .. Then, RNase Inhibitor RiboLock (1,6 U/μl) was added and 25 μL incubated with ADAR-IVTra (IP 6 , IP 5 or no IPs) protein bound magnetic beads at 27 °C for 24 h. For RT-PCR, 4 ng of IVT RNA were in vitro transcribed using the reverse primer of the respectively gene (Table S6) and QuantiTect Reverse Transcription Kit (Qiagen) or LunaScript RT Master Mix Kit (Cat. # E3025L, New England Biolabs) according to the manufacturer’s protocol. cDNA amplification was performed using the respectively primers from Table S6 and DreamTaq Green PCR Master Mix (2x) (Thermo Fisher Scientific). .. PCR product was purified with GeneJET PCR Purification Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol and Sanger sequenced using standard T7 primer (5’-TAATACGACTCACTATAGGG-3′, Seqlab, Microsynth) or the reverse primer in the case of HTR2C (Table S6).

    Article Title: Virus-Like Particles: The Next Frontier in Livestock Gene Editing
    Article Snippet: .. Therefore, RNA of wildtype and VLP-transduced organoids was isolated, and cDNA was synthesized using the LunaScript RT Master Mix Kit (NEB) according to the manufacturer’s protocol. cDNA was diluted (1:3) and the desired loci amplified with the PyroMark PCR kit (Quiagen, Hilden, GER). ..

    Polymerase Chain Reaction:

    Article Title: Inositol hexakisphosphate Functions as a Cofactor and Modulator of ADAR1 Activity
    Article Snippet: .. Then, RNase Inhibitor RiboLock (1,6 U/μl) was added and 25 μL incubated with ADAR-IVTra (IP 6 , IP 5 or no IPs) protein bound magnetic beads at 27 °C for 24 h. For RT-PCR, 4 ng of IVT RNA were in vitro transcribed using the reverse primer of the respectively gene (Table S6) and QuantiTect Reverse Transcription Kit (Qiagen) or LunaScript RT Master Mix Kit (Cat. # E3025L, New England Biolabs) according to the manufacturer’s protocol. cDNA amplification was performed using the respectively primers from Table S6 and DreamTaq Green PCR Master Mix (2x) (Thermo Fisher Scientific). .. PCR product was purified with GeneJET PCR Purification Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol and Sanger sequenced using standard T7 primer (5’-TAATACGACTCACTATAGGG-3′, Seqlab, Microsynth) or the reverse primer in the case of HTR2C (Table S6).

    Article Title: Virus-Like Particles: The Next Frontier in Livestock Gene Editing
    Article Snippet: .. Therefore, RNA of wildtype and VLP-transduced organoids was isolated, and cDNA was synthesized using the LunaScript RT Master Mix Kit (NEB) according to the manufacturer’s protocol. cDNA was diluted (1:3) and the desired loci amplified with the PyroMark PCR kit (Quiagen, Hilden, GER). ..

    Real-time Polymerase Chain Reaction:

    Article Title: ER-associated control of axonal vesicle trafficking during neuronal development
    Article Snippet: RNA quantity and purity were assessed by spectrophotometry (NanoDrop, Thermo Fisher Scientific). .. For each sample, 500 ng to 1 μg of RNA was reverse-transcribed using the LunaScript RT Master Mix Kit (New England Biolabs, E3025L), in a final reaction volume of 20 μL, according to the supplier’s recommendations. qPCR was performed using the Luna Universal qPCR Master Mix (New England Biolabs, M3003X) on a 7500 qPCR fast System (Applied Biosystems). .. Each 20 μL reaction contained 4 μL of diluted cDNA and gene-specific primers purchased from IDT DNA: Rat ActinB: Rn.PT.39a.22214838.g, Rat Lppr4 (PRG1): Rn.PT.58.19098303, Rat Lppr3 (PRG2): Rn.PT.58.35042039.

    Isolation:

    Article Title: Virus-Like Particles: The Next Frontier in Livestock Gene Editing
    Article Snippet: .. Therefore, RNA of wildtype and VLP-transduced organoids was isolated, and cDNA was synthesized using the LunaScript RT Master Mix Kit (NEB) according to the manufacturer’s protocol. cDNA was diluted (1:3) and the desired loci amplified with the PyroMark PCR kit (Quiagen, Hilden, GER). ..

    Synthesized:

    Article Title: Virus-Like Particles: The Next Frontier in Livestock Gene Editing
    Article Snippet: .. Therefore, RNA of wildtype and VLP-transduced organoids was isolated, and cDNA was synthesized using the LunaScript RT Master Mix Kit (NEB) according to the manufacturer’s protocol. cDNA was diluted (1:3) and the desired loci amplified with the PyroMark PCR kit (Quiagen, Hilden, GER). ..



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